Broad-range Glycosidase Activity Profiling

Towards broad spectrum activity - based glycosidase probes : synthesis and evaluation of deoxygenated cyclophellitol aziridines

Towards broad spectrum activity-based glycosidase probes: synthesis and evaluation of deoxygenated cyclophellitol aziridines.

Activity-based protein profiling has emerged as a powerful tool for visualizing glycosidases in complex biological samples. Several configurational cyclophellitol isomers have been shown to display high selectivity as probes for glycosidases processing substrates featuring the same configuration. Here, a set of deoxygenated cyclophellitols are presented which enable inter-class profiling of β-g...

Creation of an α-mannosynthase from a broad glycosidase scaffold.

Glycosynthases provide a rare and practical source of catalytic synthetic utility for oligosaccharide and other glycoside synthesis. These biocatalysts, nucleophile-less mutants of retaining glycosidases, are typically exquisitely stereoselective (inverting) enzymes and some can also attach monosaccharides with good regioselectivity. Many examples now exist, but in most cases b-glycosides are s...

Bacteriophage particles with endo-glycosidase activity.

Some Escherichia coli K bacteriophage particles, capable of interacting specifically with bacterial polysaccharide capsules, carry an endo-glycosidase activity, probably located in the spikes.

Clinical Utility of Broad-Range Fungal PCR